C4zip

In the world of proteomics, the quality of your mass spectrometry data is only as good as your sample preparation. Contaminants like salts, buffers, and detergents can easily drown out the signals of the very proteins you’re trying to study. This is where become indispensable. What are C4 Zip-tips?

Preparing the resin with a wetting solution (e.g., 50:50 acetonitrile in water). In the world of proteomics, the quality of

In recent patent filings and research papers, C4 Zip-tips have been critical for identifying protein isoforms and calculating theoretical masses within 0.5 Da of their primary amino acid sequences. By removing buffers and exchange salts, researchers can achieve the high-resolution mass spectra required for modern drug development and biological research. CN101883788A - 多肽，抗体可变结构域&拮抗剂 What are C4 Zip-tips

While C18 is the "gold standard" for peptide mapping, it can bind proteins so tightly that they become nearly impossible to elute. The shorter carbon chain of the C4 resin offers: By removing buffers and exchange salts, researchers can

Essential when working with low-abundance samples.

Produced by Millipore , C4 Zip-tips are 10 µL pipette tips containing a specialized C4 (butyl) stationary phase resin. Unlike the more common C18 tips used for peptides, the C4 resin is optimized for larger, more hydrophobic molecules like intact proteins and large polypeptides. Why C4 over C18?

Using these tips typically follows a simple four-step process: